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The clinical test protein acquired from a patient's serum is typically obtained through a process called immunoassay. This process involves the use of antibodies that are specific to the protein being tested. The antibodies are added to the patient's serum sample and bind to the protein, forming an antigen-antibody complex. This complex is then detected using a variety of methods, such as enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA), or immunofluorescence. The amount of the protein in the sample can then be determined by measuring the amount of the antigen-antibody complex.
All of the following must be considered when interpreting clinical findings and are too extensive to be covered on this site: